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Proteus NoEndo™ M (Mini), NoEndo™ S (Standard) and NoEndo™ HC (High Capacity) Spin Column Kits: The Gold Standard
Residual endotoxin contamination in advanced biotherapy products is an expensive and often difficult contaminant to control.
Many commercially-available protocols are unable to remove endotoxins effectively and are based on non-affinity chromatography methods e.g. Ion exchange chromatography, phase separation using Triton X-114 or require time consuming and expensive affinity steps. These costly resins are often supplied as loose resin or packed in slow gravity columns.
The Proteus NoEndo™ spin column kits offer a standardised method for high grade clearance of endotoxin from recombinant proteins, antibodies and viral vectors. These agents are increasingly being designed for therapeutic applications, hence moving them forward efficiently through in vivo studies requires pure preparations of the samples.
Endotoxin-free preparation in less than 1 hour. The yields of a gravity flow with the speed of a spin column
Next generation Proteus kits combine the quality separation you expect from gravity flow columns with the speed and ease-of-use of spin columns. Both column formats reveal a high degree of innovation! The Standard and High Capacity columns incorporate pre-packed resin cartridges utilizing our FlowGo™ technology. The Mini columns are empty columns that incorporate our proprietary SelfSeal™ membrane technology. This ensures that there is no passage of the sample through the membrane during the batch incubation at 40C or at room temperature.
NoEndo S and NoEndo HC Columns:
The proprietary FlowGo™ technology regulates sample movement through the technologically-advanced affinity resin cartridge, increasing both endotoxin removal and protein recovery. Uniquely, we offer flow rate control for endotoxin removal in a centrifuge.
NoEndo M Columns:
The NoEndo™ Mini columns incorporate a SelfSeal™ membrane technology which retains the NoEndo™ resin and sample in the batch incubation chamber. When the column spun in a low speed centrifuge, the pores of the membrane dilate and the filtered eluate is collected in the bottom of the centrifuge tube.
